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Figure 1

From: Integration-free induced pluripotent stem cells from three endangered Southeast Asian non-human primate species

Figure 1

Generation of Celebes Crested Macaque (Macaca nigra) iPSC lines. (A) Celebes Crested Macaque (Macaca nigra). (B) Formation of CM-iPSC colonies at day 20 after transduction. (C) Alkaline phosphatase (AP) staining of CM-iPSCs corresponding to B. (D) CM-iPSC colonies at passage 4. (E) Quantitative RT-PCR analysis for the expression of endogenous pluripotency markers in CM-iPSCs normalized to GAPDH and controlled to source CM fibroblasts. (F) ICC staining confirming positive expression of pluripotency markers. (G) Karyotype of CM-iPSCs demonstrating 42 chromosomes, XX; 20 GTG-banded cells scored and analysed, 28 karyograms prepared.

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