Figure 2 | Scientific Reports

Figure 2

From: Integration-free induced pluripotent stem cells from three endangered Southeast Asian non-human primate species

Figure 2

CM-iPSCs are capable of differentiation into the three germ layers and functional cardiomyocytes. (A) Differentiation of CM-iPSCs into EBs. (B) Quantitative RT-PCR analysis for the expression of pluripotency and germ layer markers in CM EBs normalized to GAPDH and controlled to source CM-iPSCs. (C) ICC staining of day 14 EBs confirming CM-iPSC differentiation into the three germ layers. (D) Quantitative RT-PCR analysis for the expression of PGC markers in CM EBs normalized to GAPDH and controlled to source CM-iPSCs. (E) Differentiation of CM-iPSCs into cardiomyocytes. (F) Quantitative RT-PCR analysis for the expression of cardiomyocyte markers in CM cardiomyocytes normalized to GAPDH and controlled to source CM-iPSCs. (G) ICC staining of CM cardiomyocytes confirming CM-iPSC differentiation into cardiomyocytes.

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