Figure 3 | Scientific Reports

Figure 3

From: Integration-free induced pluripotent stem cells from three endangered Southeast Asian non-human primate species

Figure 3

Generation and differentiation of Lar Gibbon (Hylobates lar) iPSC lines. (A) Lar Gibbon (Hylobates lar) (B) LG-iPSC colonies at passage 6. (C) Quantitative RT-PCR analysis for the expression of endogenous pluripotency markers in LG-iPSCs normalized to GAPDH and controlled to source LG fibroblasts. (D) ICC staining confirming positive expression of pluripotency markers in LG-iPSCs. (E) Karyotype of LG-iPSCs demonstrating 44 chromosomes, XX; 20 GTG-banded cells analysed, 20 karyograms prepared. (F) Quantitative RT-PCR analysis for the expression of pluripotency and germ layer markers in LG EBs normalized to GAPDH and controlled to source LG-iPSCs. (G) ICC staining of LG EBs confirming LG-iPSC differentiation into the three germ layers.

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