Figure 4 | Scientific Reports

Figure 4

From: Integration-free induced pluripotent stem cells from three endangered Southeast Asian non-human primate species

Figure 4

Generation and differentiation of Siamang (Symphalangus syndactylus) iPSC lines. (A) Siamang (Symphalangus syndactylus) (B) Alkaline phosphatase (AP) staining of SM-iPSC colonies at passage 9. (C) Quantitative RT-PCR analysis for the expression of endogenous pluripotency markers in SM-iPSCs normalized to GAPDH and controlled to source SM fibroblasts. (D) ICC staining confirming positive expression of pluripotency markers in SM-iPSCs. (E) Karyotype of SM-iPSCs demonstrating 50 chromosomes, XX; 20 GTG-banded cells analysed, 21 karyograms prepared. (F) Quantitative RT-PCR analysis for the expression of pluripotency and germ layer markers in SM EBs normalized to GAPDH and controlled to source SM-iPSCs. (G) Quantitative RT-PCR analysis for the expression of PGC markers in SM EBs normalized to GAPDH and controlled to source SM-iPSCs. (H) ICC staining of SM EBs confirming SM-iPSC differentiation into the three germ layers.

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