Figure 5 | Scientific Reports

Figure 5

From: Integration-free induced pluripotent stem cells from three endangered Southeast Asian non-human primate species

Figure 5

Generation and differentiation of Proboscis Monkey (Nasalis larvatus) iPSC lines. (A) Proboscis Monkey (Nasalis larvatus) (B) PM-iPSC colonies at passage 8. (C) Quantitative RT-PCR analysis for the expression of endogenous pluripotency markers in PM-iPSCs normalized to GAPDH and controlled to source PM fibroblasts. (D) ICC staining confirming positive expression of pluripotency markers in PM-iPSCs. (E) Karyotype of PM-iPSCs demonstrating 48 chromosomes, XY; 20 GTG-banded cells scored and analysed, 20 karyograms prepared. (F) Quantitative RT-PCR analysis for the expression of pluripotency and germ layer markers in PM EBs normalized to GAPDH and controlled to source PM-iPSCs. (G) Quantitative RT-PCR analysis for the expression of PGC markers in CM EBs normalized to GAPDH and controlled to source CM-iPSCs. (H) ICC staining of PM EBs confirming PM-iPSC differentiation into the three germ layers.

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