Table 1 Details of primers and PCR conditions used in this study.
Primers | Sequence (5’-3’) | PCR Condition | Amplicon size (bp) |
|---|---|---|---|
ompW | f-CACCAAGAAGGTGACTTTATTGTG r-GAACTTATAACCACCCGCG | 94 °C for 30 s, 64 °C for 30 s, 72 °C for 30 s | 586 |
rfb-O1 | f-GTTTCACTGAACAGATGGG r-GGTCATCTGTAAGTACAAC | 94 °C for 1 min, 55 °C for 1 min, 72 °C for 1 min | 192 |
rfb-O139 | f-AGCCTCTTTATTACGGGTGG r-GTCAAACCCGATCGTAAAGG | 94 °C for 1 min, 55 °C for 1 min, 72 °C for 1 min | 449 |
tcpA (Classica) | f-CACGATAGGAAAACCGGTCAAGAG r-ACCAAATGCAACGCCGAATCGAG | 94 °C for 1 min, 60 °C for 1 min, 72 °C for 1 min | 617 |
tcpA (El Tor) | f-AAGAAGTTTGTAAAAGAAGAACAC r-GAAAGGACCTTCTTTCACGTTG | 94 °C for 1 min, 60 °C for 1 min, 72 °C for 1 min | 471 |
toxR, | f-TGTTCGGATTAGGACAC r-TACTCACACACTTTGATGGC | 94 °C for 1 min, 60 °C for 1 min, 72 °C for 1 min | 883 |
hlyA | f-GGC AAA CAG CGA AAC AAA TAC C r-CTC AGC GGG CTA ATA CGG TTT A | 94 °C for 1 min, 60 °C for 1 min, 72 °C for 1 min | 481 |
ctx | f-CGGGCAGATTCTAGACCTCCTG r-CGATGATCTTGGAGCATTCCCAC | 94 °C for 1 min, 60 °C for 1 min, 72 °C for 1 min | 564 |
zot | f-TCGCTTAACGATGGCGCGTTTT r-AACCCCGTTTCACTTCTACCCA | 94 °C for 1 min, 60 °C for 1 min, 72 °C for 1 min | 243 |
ace | f-GCTTATGATGGACACCCTTTA r-TTTGCCCTGCGAGCGTTAAAC | 94 °C for 1 min, 55 °C for 1 min, 72 °C for 1 min | 284 |
