Figure 1 | Scientific Reports

Figure 1

From: ITGB1 and DDR activation as novel mediators in acquired resistance to osimertinib and MEK inhibitors in EGFR-mutant NSCLC

Figure 1

Hyperactivation of MAPK signaling along with EMT pathways in OR NSCLC cell lines. (a) Cell toxicity assay in parental (PC9, H1975) and resistant (PC9/OR, H1975/OR) cells exposed to increasing concentrations of osimertinib for 72 h. (b–d) Representative western blotting of whole cell lysates showing levels of phosphorylated and total forms of (b) EGFR, AKT, MEK, ERK1/2, p38, (c) expression of SNAIL and SLUG in whole cell lysates and TWIST in nuclear extracts and (d) levels of vimentin, n-cadherin, TACE and e-cadherin in both parental and OR NSCLC cell lines. Actin, tubulin and histone H3 were used to ensure equal loading. At least three independent experiments were performed. (e) Representative microscopic images and quantitative analysis of migration assay. Migrated cells (to 5% FBS) were stained with crystal violet (20× magnification) and counted. A mean of migrated cells per visual field of 3 replicate wells was graphically reported. Statistical significance **p < 0.01 and ***p < 0.001. At least three independent experiments were performed.

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