Figure 3 | Scientific Reports

Figure 3

From: ITGB1 and DDR activation as novel mediators in acquired resistance to osimertinib and MEK inhibitors in EGFR-mutant NSCLC

Figure 3

Effect of selumetinib on cell viability and migration. (a) MTS assay in PC9/OR and H1975/OR cells exposed to increasing concentrations of selumetinib for 72 h. Data are expressed as mean of three independent experiments ± SD. (b) Representative western blotting of whole cell lysates showing levels of phosphorylated and total forms of MAPKs (p-ERK1/2, ERK1/2, p-p38, p38). Actin was used to ensure equal loading. (c) Representative images (20× magnification) and quantitative analysis of untreated and treated cells migrated through the trans-well insert and stained with crystal violet. Statistical significance **p < 0.01 and ***p < 0.001. (d) Expression levels of EMT markers (vimentin and SNAIL), ITGB1, p-FAKTyr397 and FAK in response to treatment with selumetinib (2.5 µM) for 72 h. GAPDH was used as equal loading.

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