Table 1 Reagents.
From: Hyperoxia prevents the dynamic neonatal increases in lung mesenchymal cell diversity
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
Strain, strain background (M. musculus) | C57BL/6J | The Jackson Laboratory | Stock No:000664 | Â |
Commercial assay or kit | Liberase TM TL Research Grade | Sigma Aldrich | Cat No. 5401020001 | Â |
Antibody cocktail | Purified Anti-Mouse CD16/CD32 (Fc Shield; Rat monoclonal) | Tonbo Biosciences | Clone: 2.4G2 Cat No. 70-0161-U100 | Pre-FACS (1:100) |
Antibody | Anti-CD31 (Rat monoclonal) | BioLegend | Clone: MEC13.3 Cat No. 102501 | FACS (1:100) |
Antibody | Anti-CD326 Monoclonal Antibody, eFluor 450 | eBioscience/ThermoFisher | Clone: G8.8 Cat# 48-5791-82 | FACS (1:100) |
Antibody | Anti-CD45 (Rat monoclonal) | eBioscience/ThermoFisher | Clone: 30-F11 Cat#14-0451-82 | FACS (1:100) |
Commercial assay or kit | RNAscope® Multiplex Fluorescent Detection Kit v2 | Advanced cell diagnostics (ACD) | Cat No. 323110 |  |
Commercial assay or kit | Probe-Mm-Tgfbi | ACD | Cat No. 494551 | RNAscope: 50 μl per slide |
Commercial assay or kit | Probe-Mm-Pdgfra-C2 | ACD | Cat No. 480661-C2 | RNAscope: (1:50) |
Commercial assay or kit | Probe-Mm-Hhip-C3 | ACD | Cat No. 448441-C3 | RNAscope: (1:50) |
Commercial assay or kit | Probe-Mm-Crh-C2 | ACD | Cat No. 418011-C2 | RNAscope: (1:50) |
Commercial assay or kit | Probe-Mm-Mki67-C3 | ACD | Cat No. 416771-C3 | RNAscope: (1:50) |
Commercial assay or kit | Probe-Mm-Wnt2 | ACD | Cat No. 313601 | RNAscope: 50 μl per slide |
Commercial assay or kit | Probe-Mm-Tubb3-C2 | ACD | Cat No. 423391-C2 | RNAscope: (1:50) |
Commercial assay or kit | Probe-Mm-Cspg4-C3 | ACD | Cat No. 404131-C3 | RNAscope: (1:50) |
Commercial assay or kit | Probe-Mm-Acta1-C3 | ACD | Cat No. 808831-C3 | RNAscope: (1:50) |
