Figure 3
From: Hexose phosphorylation for a non-enzymatic glycolysis and pentose phosphate pathway on early Earth
Identification of glucose 6-phosphate and 6-phosphogluconate by LC–MS/MS and NMR. (a) LC–MS/MS MRM chromatograms of the experimental product of glucose phosphorylation at 80 °C and glucose 6-phosphate standard (m/z: 259 > 97). The yields represent the average of triplicated experiments. (b) Product ion spectrum of the experimental product of glucose phosphorylation in the borate-added product at 80 °C. The mass signals of m/z of 259 and 97 correspond to glucose-phosphate and phosphate, respectively. (c) Product ion spectrum of the glucose 6-phosphate standard. (d) 31P-NMR spectrum of the experimental product of glucose phosphorylation at 80 °C (see also Fig. S4). (e) LC–MS/MS MRM chromatograms of the experimental product of gluconate phosphorylation at 80 °C and 6- phosphogluconate standard (m/z: 275 > 97). The yields represent the average of triplicated experiments. (f) Product ion spectrum of the experimental product of gluconate phosphorylation in the borate-added product at 80 °C. The mass signals of m/z of 275 and 97 correspond to phosphogluconate and phosphate, respectively. (g) Product ion spectrum of the 6-phosphogluconate standard. (h) 31P-NMR spectrum of the experimental product of gluconate phosphorylation at 80 °C (see also Fig. S5).
