Figure 2 | Scientific Reports

Figure 2

From: Translational enhancement of target endogenous mRNA in mammalian cells using programmable RNA-binding pentatricopeptide repeat proteins

Figure 2

Translational enhancement of p53 mRNA by PPR-eIF4G fusion gene induces apoptosis. (A) Apoptosis activity was analyzed using a florescent probe recognizing activated caspase-3/7, with or without pP10-4G transfection. Representative microscopic bright field (BF) and fluorescent (FL) images are shown. Mock indicates transfection with the empty vector. (B) Estimation of the ratio of fluorescence-positive cells by flow cytometry. Unpaired two-tailed Student’s t-test, N = 3, ***p < 0.001. Error bar indicates the standard deviation.

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