Figure 3 | Scientific Reports

Figure 3

From: Translational enhancement of target endogenous mRNA in mammalian cells using programmable RNA-binding pentatricopeptide repeat proteins

Figure 3

Role of PPR and eIF4G domain in the translational enhancement. The translational enhancement was evaluated by transfection with pP10-4G, pP10-HA, pP10-4E, pP10-4A, and pP3-4G. (A) Cell number evaluated as shown in Fig. 1. Unpaired two-tailed Student’s t-test, N = 3, **p < 0.01. Error bar indicates the standard deviation. (B) Analysis of protein accumulation level, same as in (A). (C) Protein level with mock, pP10-HA, pP3-4G, or pP10-4G transfection examined by western blot analysis using anti-FLAG antibody.

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