Figure 6
CRISPRi knockdowns of genes that produced similar transcriptional phenotypes to LTN1 and NEMF were not directly involved in RQC function (Right). Venn diagram showing the top 10 knockdown genes with similar transcriptional responses for each RQC component and ZNF598, of which 7 genes have a similar profile to NEMF and LTN1 knockdown. (Left) Graph of GFP fluorescence showing increased fluorescence levels when the components of the RQC complex are deleted, which was not observed for any of the 7 genes with similar transcriptional profiles. Data obtained from47.
