Figure 2
(a) EIS Nyquist plots and (b) Ret values of electrodes at different modification steps in [Fe(CN)6]3−/4− (2.5 mM) and 0.1 M KCl mixed solution: (a-1) GCE, (b-1) GCE-MWCNTs-Chi, (c-1) GCE-MWCNTs-Chi-FAdV-I/MAb, (d-1) GCE-MWCNTs-Chi-FAdV-I/MAb-BSA, (e-1) GCE-MWCNTs-Chi-FAdV-I/MAb-BSA-FAdV-I. (c) and (d) CV curves of the (a-1) GCE, (b-1) GCE-MWCNTs-Chi, (c-1) GCE-MWCNTs-Chi-FAdV-I/MAb, (d-1) GCE-MWCNTs-Chi-FAdV-I/MAb-BSA, and (e-1) GCE-MWCNTs-Chi-FAdV-I/MAb-BSA-FAdV-I. The scan rate was 50 mV s−1. (e and f) Amperometric i-t curves of the immunosensor for the detection of 103.43 EID50/mL FAdV-I (a-1) blank; (b-1) MWCNTs-Chi-PtNPs-FAdV-I/PAb; (c-1) MWCNTs-Chi-AgNPs-FAdV-I/PAb; (d-1) Chi-Pt/AgNPs-FAdV-I/PAb; and (d-1) MWCNTs-Chi-Pt/AgNPs-FAdV-I/PAb as labels; (f-1) immunosensor without incubation of FAdV-I/MAb (g-1) without incubation of FAdV-I/MAb but with incubation of FAdV-I and MWCNTs-Chi-Pt/AgNPs-FAdV-I/PAb as labels at -0.1 V toward the reduction of 5 mM H2O2 in PBS (10 mL, pH 7.4). (Error bar = RSD, n = 5).
