Figure 5

(a) Amperometric i-t current response of the developed electrochemical immunosensor for detecting the following concentrations of FAdV-I at − 0.1 V vs. SCE: (a-1) 10^0.43 EID₅₀ mL⁻¹, (b-1) 10^0.93 EID₅₀ mL⁻¹, (c-1) 10^1.43 EID₅₀ mL⁻¹, (d-1) 10^1.93 EID₅₀ mL⁻¹, (e-1) 10^2.43 EID₅₀ mL⁻¹, (f-1) 10^2.93 EID₅₀ mL⁻¹, (g-1) 10^3.43 EID₅₀ mL⁻¹, and (h-1) 10^3.93 EID₅₀ mL⁻¹. (b) Calibration curve of the response of the developed electrochemical immunosensor to different concentrations of FAdV-I. Error bar = RSD (n = 5). (c) Specificity of the developed electrochemical immunosensor for the target FAdV-I and other interfering substances. (d) Reproducibility of the proposed electrochemical immunosensor in the presence of 10^3.43 EID₅₀ mL⁻¹ FAdV-I. (e) Long-term stability of the developed electrochemical immunosensor.