Figure 4

Association between proteins (mMexA-OprM) solubilized in solution (3-d configuration). Variation of diffusion coefficients \(D\) with \({r}_{mAM}={n}_{{mMexA}_{0}} /{n}_{{OprM}_{0}}\) (where \({n}_{m{MexA}_{0}}\) and \({n}_{{OprM}_{0}}\) are the input quantities of mMexA and OprM respectively) of FITC-labeled mMexA in solution in the presence of OprM at a concentration of 0.8 μM after 24 h of mixing both proteins. The high values of the diffusion coefficients indicate that mMexA does not interact with OprM. Their decrease with concentration is attributed to possible dimerization or limited aggregation of mMexA.