Figure 1 | Scientific Reports

Figure 1

From: Comparative analysis of reconstructed ancestral proteins with their extant counterparts suggests primitive life had an alkaline habitat

Figure 1

Temperature-induced unfolding curves of extant and ancestral NDKs at pH 5.0 (magenta), pH 7.0 (green) and pH 9.0 (blue). The change in ellipticity at 222 nm was monitored as a function of temperature. Within experimental error, duplicated measurements were identical. The plots were normalized with respect to the baseline of the native and denatured states. Sto, S. tokodaii NDK; Mja, M. jannaschii NDK; Tth, T. thermophilus NDK; Bha, B. halodurans NDK; Bps, B. pseudofirmus NDK; Dhy, D. hydrogenovorans NDK. NaCl indicates that the measurement was carried out in a solution containing 500 mM NaCl.

Back to article page