Table 1 Tm (°C) for ancestral and extant NDKs.

From: Comparative analysis of reconstructed ancestral proteins with their extant counterparts suggests primitive life had an alkaline habitat

Proteins

Tm (°C)

pH 5.0

pH 7.0

pH 9.0

Ancestral NDKs

 Arc3

85

111

106

 Arc4

101

106

106

 Arc5

59

106

102

 Bac3

81

107

104

 Bac4

100

100

98

 Bac5

63

105

101

Acidophilic microbial NDK

 StoNDK

107

109

98

Neutralophilic microbial NDKs

 MjaNDK

94

104

93

 TthNDK

95

98

89

Alkaliphilic microbial NDKs

 BhaNDK

58

67

62

 BhaNDK (500 mM NaCl)

53

73

70

 BpsNDK

55

65

59

 BpsNDK (500 mM NaCl)

44

69

67

 DhyNDK (500 mM NaCl)

59

73

73

  1. Tm values were estimated from the data shown in Fig. 1.
  2. Sto S. tokodaii, Mja M. jannaschii, Tth T. thermophilus, Bha B. halodurans, Bps B. pseudofirmus, Dhy D. hydrogenovorans.