Figure 2 | Scientific Reports

Figure 2

From: Effect of diet supplemented with functional amino acids and polyphenols on gut health in broilers subjected to a corticosterone-induced stress

Figure 2

Assessment of gut barrier integrity after a corticosterone challenge and the provision of a nutritional solution containing functional amino acids and polyphenols. (A) Fluorescein isothiocyanate dextran (FITC-d) plasma levels of control and treated chickens (3–5 kDa FITC-dextran gavaged at 8.32 mg/kg, n = 8 chickens/treatment at d14 and n = 12 at d32). Error bars stand for standard deviation. (B–D) Jejunum mRNA levels of gene expression at d16 and d35. Relative mRNA levels were measured by real time RT-PCR (n = 8 chickens/treatment/age). B: MUC2 (mucin 2); C: OCLN (occludin); D: ZO1 (Tight junction protein-1). Significant differences between treatments are reported (†: p < 0.1, *: p < 0.05, **: p < 0.01, ***: p < 0.001). CTRL, control group; CORT, birds challenged; CORT + MIX, birds challenged and supplemented with an amino-acid and polyphenols solution.

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