Figure 3

Time-dependent changes of the number of bacteria, BT, WBC, CRP, Presepsin and IL-6 in sepsis patients before antibiotic treatment and 24 and 72 h after. (A) Case 1: A 76-year-old woman was diagnosed with sepsis associated with urinary tract infection. The Tm mapping result (Difference Value = 0.29) was E. coli, and 2 days later, the blood culture result and urine culture result were also consistent with E. coli. Meropenem (E. coli isolates were susceptible to meropenem) was then administered systemically. Error bars indicate triplicate testing. (B) Case 2: An 88-year-old woman was diagnosed with sepsis associated with retrograde cholangitis. The Tm mapping result (Difference Value > 0.5) was polymicrobial infection, and 2 days later, the blood culture result was found to be Klebsiella oxytoca, Haemophilus influenzae, and S. pneumoniae. The blood bacterial concentration was thus calculated in conversion as E. coli. Cefepime (K. oxytoca and H. influenzae isolates were susceptible to cefepime. S. pneumoniae isolates were intermediate to cefepime) was then administered systemically. Error bars indicate triplicate testing. (C) Case 3: A 94-year-old woman was diagnosed with sepsis associated with urinary tract infection. The Tm mapping result (Difference Value = 0.19) was E. coli, and 2 days later, the blood culture result and the urine culture result were also consistent with E. coli. Tazobactam/piperacillin (E. coli isolates were susceptible to tazobactam/piperacillin) was then administered systemically. Error bars indicate triplicate testing. (D) Case 4: An 84-year-old woman was diagnosed with sepsis associated with postoperative wound infection after the spine surgery. The Tm mapping result (Difference Value = 0.28) was S. dysgalactiae, and 2 days later, the blood culture result was also consistent with S. dysgalactiae. Tazobactam/piperacillin (S. dysgalactiae isolates were susceptible to tazobactam/piperacillin) was then administered systemically. Error bars indicate triplicate testing. (E) Case 5: An 81-year-old woman was diagnosed with sepsis associated with urinary tract infection. The Tm mapping result (Difference Value = 0.48) was Enterobacter aerogenes, and 2 days later, the blood culture result and the urine culture result were also consistent with E. aerogenes (2 types of mutant strains). Tazobactam/piperacillin (E. aerogenes isolates were susceptible to tazobactam/piperacillin) was then administered systemically. Error bars indicate triplicate testing. (F) Healthy Control: A 44-year-old woman who does not have any illnesses including infectious diseases and participated in this study as a volunteer. No bacteria were detected in the blood using the Tm mapping method and blood culture. Error bars indicate triplicate testing.