Figure 2

Gene expression analysis of E-cadherin, N-cadherin, and vimentin using qPCR for MCF-7 or MDA-MB-231 cells treated with the extracellular metabolites released from (A–F) MDA-MB-231 cells or (G–L) MCF-7 cells and extracted using hexane, DCM, or ethyl acetate compared to untreated cells (control). Metabolites extracted refer to the cell type from which the metabolites were extracted. Treated cells refer to the type of cells that are treated with metabolite extracts. All treatments were performed in triplicates. Changes in the gene expression is expressed as gene fold change. Data were presented as mean ± SEM (n = 3). The analysis was performed using one-way analysis of variance (ANOVA) and Tukey’s multiple comparison test. P value ≤ 0.05 was considered significant. * Reveals that P value < 0.05, ** reveals that P value < 0.01, *** reveals that P value < 0.001, **** reveals that P value < 0.0001.