Figure 3 | Scientific Reports

Figure 3

From: 1H-NMR metabolomics analysis identifies hypoxanthine as a novel metastasis-associated metabolite in breast cancer

Figure 3

Metabolite distribution in MCF-7 and MDA-MB-231 conditioned media. (A) Venn diagram showing the number of metabolites in ethyl acetate, DCM, and hexane fractions in MCF-7 and MDA-MB-231 conditioned media. (B) Unsupervised hierarchical clustering and heatmap analysis of extracellular metabolites released from MDA-MB-231 and MCF-7 cells extracted using ethyl acetate fractions. The values of metabolites represent the average concentration (mM) in MCF-7 or MDA-MB-231 conditioned media. Different colors represent the concentration of different metabolites, where red represents a high concentration and blue represents a low concentration. (C) PCA of the metabolite profiling of MCF-7 and MDA-MB-231 conditioned media extracted using ethyl acetate. The red color indicates MDA-MB-231, and green indicates MCF-7 conditioned media. (D) VIP score plot of MCF-7 and MDA-MB-231 conditioned media metabolites. (E) Metabolite set enrichment analysis (MSEA) ranked by Holm P value showed the top 25 enriched pathways by ethyl acetate fraction of MDA-MB-231 conditioned media.

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