Figure 7
Hypoxanthine-associated mechanisms to induce EMT, migration and invasion in MCF-7 cells. (A) Snail gene expression analysis using qRT-PCR. (B) Snail protein expression using western blot. (C) The luminescence of H2O2 (ROS) released from MCF-7 cells treated with different concentrations of hypoxanthine. (D) MMP-2 protein expression using western blot. (E) HIF-1α gene expression analysis using qRT-PCR. (F) HIF-1α protein expression using western blot. (G) PCSK-9 gene expression. (H) VEGF gene expression. (I) PDGF gene expression analysis using qRT-PCR. Data were presented as mean ± SEM (n = 3). The data were analyzed using one-way ANOVA) and Tukey’s multiple comparison test. P value ≤ 0.05 was considered significant. * Reveals that P value < 0.05, ** reveals that P value < 0.01, *** reveals that P value < 0.001, **** reveals that P value < 0.0001. Original blots are presented in Figs. S10 and S11.
