Figure 2
From: Direct effects of adipocyte lipolysis on AMPK through intracellular long-chain acyl-CoA signaling
Activation of AMPK through direct stimulation of lipolysis requires ATGL. (A) Representative western blot images for p-ACC S79, total ACC, p-AMPKα T172 and total AMPKα following a 30 min pretreatment with either dimethyl sulfoxide (DMSO) control, or Atglistatin (ATGLi; 20 μM) and then a 1 h treatment with lipolysis activator SR-3420 (20 μM) or DMSO. Quantified phospho/total ratios for ACC (B) and AMPK (C) from experiments in (A). (D) Glycerol measured in treatment media following 30 min pretreatment with ATGLi and/or 1 h treatment with SR-3420. Data are from n = 3 independent experiments with 2–3 replicate wells per experiment. *p > 0.05, **p > 0.01 and ***p > 0.001 as determined by one-way ANOVA with Šidák’s multiple comparison test.
