Figure 4

Visual representation of the effect of ‘Target gene’ on the reported Cq values as probability distributions. The X-axis depicts the spread in predicted Cq value per target gene (Y-axis) relative to the mean Cq value of all target genes combined. The median (black dot) and 50% and 95% distribution intervals (black lines) are indicated per target gene. The total number of datapoints for each target gene is shown in brackets. The different sizes of datasets are displayed using light blue (range < 20), blue (range 20–99) and dark blue (100+).