Figure 1 | Scientific Reports

Figure 1

From: Understanding the molecular mechanism of pathogenic variants of BIR2 domain in XIAP-deficient inflammatory bowel disease

Figure 1

Mutant XIAP BIR2 with non-synonymous PVs failed to interact with RIP2. (a) Co-IP of GFP-tagged XIAP WT or mutants, and RFP-tagged RIP2 confirmed their interaction. The tagged XIAP was immunoprecipitated from lysates of HEK 293 T cells transfected with plasmids encoding the indicated GFP-tagged XIAP WT or mutants, and western blot images were cropped for clarification. (b) Comparison of relative correlation amplitudes of live HEK 293 T cells expressing the indicated XIAP mutants. The relative correlation amplitudes between GFP–XIAP mutants and RFP-RIP2 were determined by confocal imaging during FCCS. The negative control indicates a mean value of relative correlation amplitude of 0, obtained from cells co-expressing EGFP and RIP2. The mean amplitudes of XIAP mutants were significantly lower than that of the positive control (XIAP-WT + RIP2).

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