Figure 6
Relative abundance and distribution of sialylated N-glycans (bladder n = 4, breast n = 7, cervix n = 4, colon n = 10, esophagus n = 5, gastric n = 4, kidney n = 3, liver n = 4, lung n = 10, sarcoma n = 4, skin n = 5, pancreas n = 5, prostate n = 8, thyroid n = 6, uterus n = 3). Significance is marked as follows: (*): p-value < 0.05; (**): p-value < 0.01; (***): p-value < 0.001; (****): p-value < 0.0001. Error bars on bar charts represent one standard deviation. Error bars on boxplots represent the quartiles. (A) Linkage-specific amino groups are added to sialic acid with “CLICK chemistry” to stabilize sialylation and distinguish between α2,6 and α2,3 linkages. (B) Total ion count of sialylated N-glycan relative abundance by tissue type. (C) Comparison of α2,6 and α2,3 linkage totals by tissue type. (D) Average relative intensities of the top ten sialylated N-glycans in each tissue by linkage type. Each column shows the top ten sialylated N-glycans for that tissue, with the grey portion representing the sum of remaining N-glycans. Legend shows glycan structures and glycans are arranged by sialic acid linkage type in a gradient from smallest m/z to largest. (E) Overall relative proportion of sialic acid linkages seen in each tissue type. (F) Comparison of α2,6 linkage relative abundances between normal and cancerous tissues. (G) Comparison of α2,3 linkage relative abundances between normal and cancerous tissues. (H) Comparison of mixed α2,6/α2,3 sialylation between normal and cancerous tissues of each type.
