Figure 2
Brillouin imaging of the healthy bladder wall. (A) Typical Brillouin spectrum of water obtained with our Brillouin microscope. The spectrum is composed by 2 Rayleigh peaks of adjacent dispersion orders (fixed at 0 and FSR = 30 GHz), and 4 Brillouin peaks whose location is clarified underneath the x-axis. In backscattering configuration and λ = 532 nm, water νB = 7.40 GHz. (B) DIC image of murine bladder wall. Example of 4 Brillouin spectra obtained from different regions of a bladder section. U: urothelium, L: lamina propria, M: muscle, buffer is PBS. (C) Bladder wall imaging by different microscope techniques. Scale bars = 20 µm. Upper panel: HE staining of the healthy rat bladder wall, showing the different anatomical bladder tissue layers, as in Fig. 1. Lower panel: DIC image and corresponding Brillouin maps of healthy bladder walls from urothelium (left) to muscle (right). FSR: Free Spectral Range. DIC: differential interference contrast microscopy. HE: hematoxylin eosin staining. AU: arbitrary unit.
