Figure 5
Comparison between Brillouin imaging and AFM-based microindentation experiments performed on the same bladder samples. (A) Brillouin Microscopy (left column) and microindentation-based (right column) imaging of murine bladder walls belonging to healthy (upper panel) and 4 months after X-ray (lower panel) rats. Investigation of mechanical properties of adjacent slides belonging to the same bladder, whose sample was prepared following the same protocol. We also report H&E staining sections of the same tissues performed after biomechanical acquisitions. The gradient curve of each map is shown alongside, depicting the mean ± SD across the section width. Respective color bars for each map are shown at the bottom. (B) Fold change ratio of Longitudinal and Young Modulus over time in the different layers. Q1-4: Quadrant number. Young’s modulus values here plotted have been adapted from this previously published study14 .
