Figure 2
Mapping the enzymes used in carbohydrate (HMO) digestion to the full complement of simulated glycoside hydrolase enzymes available to an organism. (A) Krona diagram showing the GH enzyme profiles on the inner ring, which reflect simulated digestion of a heterogeneous population of HMOs, map to one or more profiles of available enzymes in the outer ring, which corresponds with the bacterial species. (B) Unique enzyme profile values belonging to the six named spaces in (A), Bifidobacterium bifidum, Bifidobacterium longum, Clostridium butyricum, Clostridium perfringens, Oribacterium sinus and Treponema succinifaciens. The enzyme profile value is illustrated by a vector of cells (1 to 13, left to right, representing the enzymes of Table 1) filled according to a colour representing the substituent removed, or bond cleaved: red (l-fucose); yellow (d-galactose); orange (GalNAc); blue (GlcNAc/HexNAc) magenta (sialic acid); grey (lacto-N-biose); white (no activity). A complete set of enzyme profile values is shown in Fig. 9, while interactive Krona diagrams are provided in Supplementary Information (Krona), in which each segment can be expanded to reveal the species associated with each profile.
