Figure 7
Protective efficacy against pre-pandemic virus infection in vaccinated mice. Immunologically naïve DBA/2J mice (n = 11 per group) were vaccinated i.m. three times at four-week intervals heptavalent (blue line) formulation of COBRA recombinant HA and NA proteins, with AddaVax™ as adjuvant. Four weeks after final vaccination, mice were intranasally infected with (a, b) lethal dose of A/Sichuan/26221/2014 (106 PFU) or (c, d) lethal dose of A/Anhui/01/2013 (102 PFU), or (e, f) A/mallard/Minnesota/AI08-3437/2008 (104 PFU) in 50 µL volume. The animals were observed for clinical signs and their body weight was recorded daily post infection.
