Figure 1
From: Genetically engineered HEK cells as a valuable tool for studying electroporation in excitable cells
S-HEK cells are able to generate APs whereas NS-HEK are not. (a,b) A monolayer of S-HEK (spiking HEK) cells, a brightfield (a) and a fluorescence image (b), the membranes are labelled with the ElectroFluor630 potentiometric probe. (c,d) A single 100 µs, 150 V/cm electric pulse can trigger AP (as determined by a membrane fluorescence signal from ElectroFluor630, combined from all the cells in the field of view) in S-HEK cells (c) but not in NS-HEK cells (d). Pulse was delivered at time zero. There is a delay between the pulse delivery and the onset of AP in Fc, which is analysed further in Fig. 3f.
