Figure 2:
From: Genetically engineered HEK cells as a valuable tool for studying electroporation in excitable cells
(a) Experiment timeline. Each sample was exposed to a sequence of eight consecutive 100 µs electric pulses of different electric field strength (E ≈ 126–400 V/cm) applied 2 min apart. The arrows represent each pulse application. (b,c) Triggering changes in TMV in excitable S-HEK (b) and non-excitable NS-HEK cells (c) with the pulse sequence (results from a representative sample, only TMVs after selected E are shown, ElectroFluor630 fluorescence signal was combined from all the cells in the field of view). Pulses were delivered at time zero. (d,e) The experiments with different responses to electric pulses in S-HEK (d) and NS-HEK (e) were counted and expressed as % of all experiments. Number of experiments: N = 20 for both cell variants.
