Figure 2

Identifying signaling pathways regulating ADAMTS13 expression and the notable angiogenic markers in serum-deprived WJ-MSCs. WJ-MSCs were cultured for 48 h under serum-deprived condition, in the presence of 10 μM of the p38 pathway inhibitor, SB203580 and 10 μM of the JNK pathway inhibitor, SP600125, individually. (a) mRNA expression pattern of ADAMTS13 was analysed in the presence or absence of the inhibitors by qRT-PCR (n = 5). (b) Protein expression pattern of ADAMTS13 with p38 and JNK pathway inhibitions was detected by Western Blotting, representative image is shown (n = 4). Band intensity of protein expression levels of ADAMTS13 was quantified relative to GAPDH, which was used as a loading control and plotted. (c) mRNA expression pattern of the key angiogenic markers was analysed by qRT-PCR with p38 and JNK pathway inhibitions (n = 3). GAPDH was used as the endogenous control to normalize the gene expression levels in all the qRT-PCR studies. Serum-deprived condition has been denoted as no-serum (NS) and control as CT in the figure. Each bar represents mean ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001). Statistical comparisons were assessed using one-way ANOVA followed by Bonferroni’s multiple comparison test. Original blots are presented in Supplementary Fig. S7c.