Figure 1
From: miR-195b is required for proper cellular homeostasis in the elderly
miR-195 expression analyses. In situ hybridization of miR-195 expression (panel A, E and F) and negative controls (B, C and D) in E9.5 (panels A, B) and E12.5 (panels C–F) mouse embryos. e′ and e′′ are close ups of panel (E). f′ and f′′ are close ups of panel (F). Sequence comparison of the pre-miRNAs of miR-15/miR-16/miR-195 family members (panel G), highlighting in red the seed sequence of each mature microRNAs. RT-qPCR analyses of miR-15/miR-16/miR-195 family members in E16 embryonic (panel H) and 6 months old adult (panel I) mouse tissues. Note that miR-195a, and to a lesser extend miR-195b, are widely expressed in both embryonic and adult tissues. n = 6 in all in situ hybridization experiments. n = 3 in all RT-qPCR experiments.
