Figure 4

Effect of nsPEF on the inactivation of Pan02 cells in vitro. Cells at a concentration of 5 × 106 cells/mL in a 0.1 cm gap cuvette were treated with different doses of nsPEF: control (0), 15, 20, 30, 60, 90 pulses. (a) Cell viability measured by WST-1 metabolic activity assay at 24 h after treatment and (b) normalized TER measured for 48 h after treatment. (
Control,
15 pulses,
30 pulses,
60 pulses,
90 pulses). Mean ± SD; n = 3–5; **p < 0.01; ***p < 0.001; ****p < 0.0001.