Figure 5 | Scientific Reports

Figure 5

From: Hsa_circ_0081065 exacerbates IH-induced EndMT via regulating miR-665/HIF-1α signal axis and HIF-1α nuclear translocation

Figure 5The alternative text for this image may have been generated using AI.

Hsa_circ_0081065 exacerbates HIF-1α nuclear translocation. (A) Possible binding site between Hsa_circ_0081065 and HIF-1 α was predicted by using CATRAPID; (B) RIP assay was used to detect the hsa_circ_0081065 enrichment enriched with HIF-1α antibody; (C) RNA EMSA assay was used to detect the binding between hsa_circ_0081065 and HIF-1α protein treatment with hsa_circ_0081065 RNA probe or and HIF-1α protein; (D) Western blot was used to detect the expression of HIF-1α treatment with intermittent hypoxia and with hsa_circ_0081065 silence in IH HUVEC cells; (E) IF was used to detect the HIF-1αnuclear translocation treatment with intermittent hypoxia and overexpression of hsa_circ_0081065. **P < 0.01, ***P < 0.001; ****P < 0.0001.

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