Table 2 Expression stability ranking of the candidate reference genes according to BestKeeper, NormFinder, geNorm, Delta CT and RefFinder analyses.
Ranking | BestKeeper | NormFinder | geNorm | Delta CT | RefFinder | |||||
|---|---|---|---|---|---|---|---|---|---|---|
Gene | SD value | Gene | Stability value | Gene | M value | Gene | Average of SD | Gene | Geomean of ranking values | |
1 | fabD | 0.484 | rpoB | 0.315 | rpoB/rpoD | 0.519 | rpoB | 0.923 | rpoB | 1.41 |
2 | rpoD | 0.522 | rpoD | 0.430 | rpoB/rpoD | 0.519 | fabD | 0.943 | rpoD | 1.86 |
3 | groEL | 0.55 | fabD | 0.448 | fabD | 0.559 | rpoD | 0.968 | fabD | 2.06 |
4 | rpoB | 0.60 | gyrA | 0.465 | gyrA | 0.671 | gyrA | 0.971 | gyrA | 4.23 |
5 | gyrA | 0.645 | gapA | 0.751 | gapA | 0.738 | gapA | 1.092 | gapA | 5.44 |
6 | rho | 0.748 | proC | 0.804 | proC | 0.787 | proC | 1.138 | proC | 6.64 |
7 | gapA | 0.814 | rho | 0.812 | rho | 0.857 | rho | 1.169 | rho | 6.74 |
8 | atpD | 0.898 | atpD | 1.016 | atpD | 0.928 | atpD | 1.284 | groEL | 6.84 |
9 | proC | 0.986 | groEL | 1.069 | groEL | 0.996 | groEL | 1.327 | atpD | 8.0 |
10 | 16S | 1.534 | 16S | 1.711 | 16S | 1.166 | 16S | 1.845 | 16S | 10.0 |
