Figure 2

(A) Average stomatal aperture width overtime in lettuce plants following mock treatment and maintained vertically as a control (0 RPM) or rotated at 2/4 RPM. (B) Average stomatal aperture width overtime in lettuce plants following inoculation with S. typhimurium maintained vertically as a control (0 RPM) or rotated at 2/4 RPM. Stomatal apertures were imaged with confocal microscopy and aperture size was measured using ImageJ. At each timepoint, 3 leaves were harvested (one per plant), and 25 stomata were measured from each leaf. This was repeated in two experimental replicates (n = 150 stomata total per timepoint). Letters indicate statistical similarity between timepoints within a treatment determined using an all-pairs Tukey HSD test (p < 0.05).