Figure 4

AlloP has little effect on autophagy in astrocytes. (a) Primary rat cortical astrocytes in phase contrast, GFP, and RFP channels at 10× after a 24 h treatment with 500 nM Torin1 and 10 µM AlloP. Scale bar: 25 µm. (b) Background-subtracted mean fluorescence ratios relative to their vehicle-treated control. Each normalized point represents a 35 mm dish (3–8 cells analyzed per dish; 6 independent experiments). Lines connect independent dishes treated during the same experiment. A one-way ANOVA (F (2, 15) = 15.98) showed a main effect of treatment, p = 0.0002, and Dunnett’s multiple comparisons revealed a difference between control and Torin1 (***p = 0.0001).