Figure 5
From: Early involvement of peripherally derived monocytes in inflammation in an NMO-like mouse model
Microglia are activated in early NMO lesions, and monocyte depletion further amplifies this activation. (A) Schematic design for microglia sorting and RNA-seq analysis. (B–E) Depict microglial transcriptome analysis in NMO, while (F–H) illustrate changes in microglial transcriptome following clodronate treatment. (B) A heat map shows DEGs between Normal and NMO (fold change = 1.2, p < 0.05). (C) A heat map and a volcano plot related to various microglia-specific marker of DEGs for NMO vs. Normal, NMO + CL vs. Normal and NMO + CL vs. NMO (fold change = 1.2, p < 0.05). Significantly expressed microglial homeostatic markers are highlighted. (D) Gene ontology analysis of the upregulated 480 DEGs were inferred. (E) The heat map represent the expression of chemokine/cytokine, complement and TNF singling pathway-related genes using average normalized data (log2). (F) Volcano plot represent the expression of scarvenger receptors. (G) A Venn diagram shows the overlap of upregulated genes in NMO + CL vs. NMO and NMO + CL vs. Normal. (H) GO analysis of the 217 overlapping upregulated genes between NMO + CL vs. NMO and NMO + CL vs. Normal. (I) A heat map is used to represent the expression of chemokine/cytokine, complement, oxidative stress, and inflammation-related genes within the 217 overlapping upregulated genes.
