Table 1 Differential expression of selected mRNAs involved in proliferation and cell cycle regulation in Reg-2-overexpressing vs control U251-MG cells based on RNAseq analysis.

CYCLINS

CCNE2

Essential for the control of the cell cycle at the late G1 and early S phase

ENSG00000175305

 − 2.11

0

0

CCNE1

Essential for the control of the cell cycle at the G1/S transition

ENSG00000105173

 − 1.43

6.19E–06

8.74E–05

CCND1

Regulates the cell-cycle during G1/S transition

ENSG00000110092

 − 1.87

0

0

CCND3

Regulates the cell-cycle during G1/S transition

ENSG00000112576

 − 1.31

1.61E–04

1.61E–03

CCNB1

Essential for the control of the cell cycle at the G2/M transition

ENSG00000134057

 − 1.21

7.40E–03

0.05

CCNB2

Essential for the control of the cell cycle at the G2/M transition

ENSG00000157456

 − 1.21

7.12E–03

0.04

CCNA2

Controls both the G1/S and the G2/M transition phases of the cell cycle

ENSG00000145386

 − 1.26

9.99E–04

8.13–03

CCNG2

Negative regulation of cell cycle progression

ENSG00000138764

 − 1.3

4.36E–04

3.94E–03

KINASES

CDKN2D

Inhibition of CDK4 and CDK6

ENSG00000129355

 − 1.6

4.31E–10

1.26E–08

CDK6

Serine/threonine kinase involved in the control of the cell cycle; promotes G1/S transition

ENSG00000105810

 − 1.48

1.88E–08

4.28E–07

CDK2

Serine/threonine kinase involved in the control of the cell cycle; Acts at the G1/S transition and modulates G2 progression

ENSG00000123374

 − 1.42

1.60E–13

7.23E–12

CDK11B

Plays multiple roles in cell cycle progression, cytokinesis and apoptosis

ENSG00000248333

 − 1.21

0.01

0.06

AURKB

Serine/threonine kinase, component of the chromosomal passenger complex (CPC) that acts as a key regulator of mitosis

ENSG00000178999

 − 1.2

0.01

0.07

AURKA

Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression

ENSG00000087586

 − 1.18

0.02

0.09

CDK7

Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription

ENSG00000134058

1.28

6.39E–04

5.53E–03

CDK1

Catalytic subunit of the kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of cell cycle

ENSG00000185324

 − 1.03

0.66

0.82

PLK1

Critical regulator of cell cycle progression, mitosis, cytokinesis, and the DNA damage response

ENSG00000166851

 − 1.37

1.81E–05

2.28E–04

Cell division control proteins

CDC6

Participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated

ENSG00000094804

 − 1.66

1.24E–12

4.98E–11

CDC14B

Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint

ENSG00000081377

 − 1.45

1.29E–06

2.12E–05

CDC25A

Required for progression from G1 to the S phase of the cell cycle

ENSG00000164045

 − 1.4

3.04E–06

4.58E–05

CDC20

Regulatory protein interacting with several other proteins at multiple points in the cell cycle

ENSG00000117399

 − 1.37

9.13E–06

1.24E–04

CDC25B

Phosphatase that activates the cyclin dependent kinase CDC2; required for entry into mitosis

ENSG00000101224

 − 1.23

2.47E–05

3.02E–04

CDC27

Component of the anaphase promoting complex/cyclosome (APC/C), that controls progression through mitosis and the G1 phase of the cell cycle

ENSG00000004897

 − 1.2

9.44E–03

0,056,301

CDCA8

Component of the chromosomal passenger complex that is an essential regulator of mitosis and cell division

ENSG00000134690

 − 1.17

0,025,054

0,12,479

CDC23

Essential for cell cycle progression through the G2/M transition

ENSG00000094880

 − 1.17

1.65E–03

0,012,551

CDC45

Required for initiation of chromosomal DNA replication

ENSG00000093009

 − 1.11

0,138,836

0,415,178

CDC14A

Dual-specificity phosphatase; required for centrosome separation and cytokinesis during cell division

ENSG00000079335

1.19

0,046,695

0,200,883

CDC7

Kinase that phosphorylates critical substrates regulating the G1/S phase transition and initiation of DNA replication

ENSG00000097046

1.25

6.56E–06

9.19E–05

CDC26

Component of the anaphase promoting complex/cyclosome (APC/C), that controls progression through mitosis and the G1 phase of the cell cycle

ENSG00000176386

1.3

2.66E–04

2.53E–03