Figure 1 | Scientific Reports

Figure 1

From: Two-substrate enzyme engineering using small libraries that combine the substrate preferences from two different variant lineages

Figure 1

Lineages of E. coli TK variants. Multiple phases of rational, and semi-rational design guided by structure, bioinformatics and molecular dynamics simulations have introduced mutations that altered enzyme stability and the specificity for both aldol acceptor and ketol donor substrates. The 3M lineage first achieved activity on 3-FBA but with hydroxypyruvate as donor. The 6M lineage shown at the top right corner, was developed on acceptance of pyruvate as the donor, but achieved only low activity with 3-FBA. The current work explored combining the 3M and 6M lineages to improve activity of the reaction with 3-FBA and pyruvate.

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