Table 1 Specific activities of previous variants towards aromatic aldehydes.

From: Two-substrate enzyme engineering using small libraries that combine the substrate preferences from two different variant lineages

Variant

Donor

Acceptor

kcat

(/s)

Specific activity

(µmol/mg min)

References

3M: S385Y/D469T/R520Q

HPA

3-FBA

7.0

2.3

5

3M: S385Y/D469T/R520Q

HPA

4-FBA

n.d.

1.5

5

3M: S385Y/D469T/R520Q

HPA

3-HBA

n.d.

0.09

5

S385pCNF/D469T/R520Q

HPA

3-HBA

0.6

0.047

33

6M: H100L/H192P/A282P/I365L/D469T/G506A

PA

3-FBA

0.2

0.048

4

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