Figure 5

Estimation of irradiated blood volume. In (a) and (b) a photograph of a mouse ear is shown. The irradiation field is marked as indicated. In (a) the blood vessels are marked in three colors according to the evaluation. Blue indicates thick blood vessels, green middle thick blood vessels and gray thin blood vessels. From the length and the diameter, the static volume was determined and by measuring the thickness of the entry veins, the blood flow volume was calculated. In (b), the raw image is shown. In (c), the results of the estimated irradiated volumes and of the static volume are shown. The irradiated volume was estimated for a dose of 33 Gy for the three irradiation modes (Flash930, Flash9 and Conv) used in this experiment. The error bars show the standard deviation of the mean and two stars indicate p-values < 0.0004 (ANOVA, one-way, Origin 2021b). For a better visualization, the y-axes was interrupted between 2.1 µl and 10 µl with larger spaces between 0 µl and 2.1 µl.