Figure 2 | Scientific Reports

Figure 2

From: ATF4 expression in thermogenic adipocytes is required for cold-induced thermogenesis in mice via FGF21-independent mechanisms

Figure 2

ATF4 BAT KO mice have reduced BAT mass and mildly impaired glucose tolerance at baseline conditions. Data collected under baseline conditions in ATF4 BKO (KO) male mice and their respective WT littermate controls. (A) Body mass. (B) Total lean mass. (C) Total fat mass. (WT n = 8; KO n = 6). (D) Brown adipose tissue (BAT) mass normalized to body mass (n = 8). (E) UCP1 protein levels in BAT normalized to GAPDH and respective densitometric quantification (WT n = 11; KO n = 8). Images cropped from the same blot. Optical density (OD). Original immunoblots are presented in Supplemental Figure S6. (F) Core body temperature measured with a rectal probe (WT n = 8; KO n = 5). (G) Glucose tolerance test (GTT). (H) Area under the curve for the GTT. (I) Fasting glucose levels (after a 6-h fast) (WT n = 11; KO n = 12). (J) Liver triglyceride levels (WT n = 10; KO n = 7). Data are expressed as means ± SEM. Significant differences were determined by Student's t‐test, using a significance level of P < 0.05. *p < 0.05 significantly different vs. WT mice.

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