Figure 1

(A) Cu isotope fractionation (\(\Delta\)65Cu = \(\delta\)65Cubound − \(\delta\)65Cufree) due to ligation by cysteine (Cys), histidine (His), glutamate (Glu) and aspartate (Asp). Gray points represent \(\Delta\)65Cu calculated for independent experiments; black points represent the mean \(\Delta\)65Cu of replicates (± 2SE). (B–E) Molecular structures of the amino acids investigated in this study.