Figure 5

BMSCs can phagocytose and process myelin debris via the lysosomal pathway. (A) Immunofluorescence labeling was used to assess the phagocytic capacity of BMSCs (GFP, green) in the hBcl2 group to Dil-myelin (red, 500 μg/mL). Under a high-powered microscope, GFP and Dil labeling revealed that BMSCs in the hBcl2 group could phagocytize myelin. (B) Cellular immunofluorescence staining was used to assess changes of Lamp1 (green) in BMSCs following phagocytosis of Dil-myelin (red, 500 μg/mL). The number of cells is shown by DAPI. After phagocytosis of myelin, Lamp1 expression rose considerably in all BMSCs. (C) After adding Dil-myelin (red, 500 μg/mL) for 1 day, a double fluorescence staining was used to determine the phagocytosis of Dil-myelin (red, 500 μg/mL) by cells in the hBcl2, cb, NC, and BMSCs groups. (D) Quantitative analysis of the proportion of cells with Dil-myelin (red, 500 μg/mL) phagocytized by BMSCs in each group accounting for 1/3 or more of the total. (At least three independent replicates were performed for each experiment. Data are presented as mean ± SD, NS, no statistical difference, analyzed by one-way ANOVA), scale bar = 100 μm (A), scale bars = 20 μm (ROI, C), scale bar = 10 μm (B).