Figure 2 | Scientific Reports

Figure 2

From: PSGL-1 decorated with sialyl Lewisa/x promotes high affinity binding of myeloma cells to P-selectin but is dispensable for E-selectin engagement

Figure 2

Downregulation of PSGL-1 inhibits rolling and adhesion on P-selectin but not on E-selectin. MM1SHeca452 cells stably expressing a scramble shRNA control or the shRNA for PSGL-1 were tested in a rolling and adhesion assay under shear stress on recombinant E-selectin (A) and P-selectin (B), both at 15 µg/ml. Histograms represent the mean + sem of three independent experiments performed in duplicate. Symbols on top of the error bars represent the statistical significance of the rolling (white) or adhesion (grey) fractions compared to the same fractions in the scramble control. Symbols on top of the horizontal bars represent the statistical significance of the total fraction (rolling plus adhesion fraction). The unpaired one-tailed t test was used to determine statistical significance. **p < 0.01; *p < 0.05; ns non-significant.

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