Figure 3
PSGL-1 neutralization with a blocking antibody inhibits rolling and adhesion on P-selectin but not on E-selectin. MM1SHeca452 (A,C) and RPMI8226Heca452 (B,D) cells were pre-incubated for 1 h with 10 µg/ml of an anti-PSGL-1 blocking antibody or matched-isotype control and then tested in a rolling and adhesion assay under shear stress on recombinant E-selectin (A,B) and P-selectin (C,D), both at 15 µg/ml. Histograms represent the mean + sem of three independent experiments performed in duplicate. Symbols on top of the error bars represent the statistical significance of the rolling (white) or adhesion (grey) fractions compared to the same fractions in the isotype control. Symbols on top of the horizontal bars represent the statistical significance of the total fraction (rolling plus adhesion fraction). The unpaired one-tailed t test was used to determine statistical significance. ***p < 0.001; **p < 0.01; ns non-significant.
